CREB1 Rabbit Polyclonal Antibody
cat.: R1309-14
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human CREB1. |
| Positive control: | A431 cell lysate, PC12 cell lysate, Hela, human brain, mouse brain |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:200-1:500 1:200 |
| Uniprot #: | SwissProt: P16220 Human |
| Alternative names: | Active transcription factor CREB cAMP response element binding protein 1 cAMP response element binding protein cAMP responsive element binding protein 1 cAMP-responsive element-binding protein 1 CREB CREB-1 CREB1 CREB1_HUMAN Cyclic AMP-responsive element-binding protein 1 MGC9284 OTTHUMP00000163864 OTTHUMP00000163865 OTTHUMP00000206660 OTTHUMP00000206662 OTTHUMP00000206667 Transactivator protein |
Images
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Fig1: Western blot analysis on A431 (1) and PC12 (2) cell lysates using anti-CREB1 rabbit polyclonal antibodies. |
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Fig2:
Western blot analysis of CREB1 on different lysates with Rabbit anti-CREB1 (R1309-14) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si CREB1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. R1309-14 was shown to specifically react with CREB1 in Hela-si NT cells. Weakened band was observed when Hela-si CREB1 sample was tested. Hela-si NT and Hela-si CREB1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1309-14, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunofluorescent staining of Hela cells using anti- CREB1 rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- CREB1 rabbit polyclonal antibody. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- CREB1 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.