TESPA1 Rabbit Polyclonal Antibody
cat.: R1309-16
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TESPA1 aa 221-270 / 521 (F249E). |
| Positive control: | Human thymus tissue lysate, mouse thymus tissue lysate, human liver tissue lysate, A431 cell lysate, A172, rat brain, human lung cancer, mouse brain, HepG2. |
| Subcellular location: | Cytoplasm. Membrane, Endoplasmic reticulum, Signalosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:200 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: A2RU30 Human | Q3U132 Mouse |
| Alternative names: | HSPC257 K0748_HUMAN KIAA0748 Protein TESPA1 TESPA1 – thymocyte expressed, positive selection associated 1 TESPA1 thymocyte expressed, positive selection associated 1 Uncharacterized protein KIAA0748 |
Images
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Fig1: Western blot analysis of TESPA1 on different lysates using anti-TESPA1 antibody at 1/1,000 dilution. |
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Fig2: ICC staining TESPA1 in A172 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TESPA1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of HepG2 cells with TESPA1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.