Cdk2 Rabbit Polyclonal Antibody
cat.: R1309-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cdk2 aa 249-298 / 298. |
| Positive control: | A549 cell lysate, F9 cell lysate, PC-12 cell lysate, Mouse thymus tissue lysate, Human liver tissue lysate, A549, human tonsil tissue, human breast cancer tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleus, Cytoskeleton, Endosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P24941 Human | P97377 Mouse | Q63699 Rat |
| Alternative names: | Cdc2 related protein kinase cdc2-related protein kinase CDC28 CDC2A Cdk 2 CDK1 CDK2 CDK2_HUMAN CDKN2 Cell devision kinase 2 Cell division protein kinase 2 Cyclin dependent kinase 2 cyclin dependent kinase 2-alpha Cyclin-dependent kinase 2 kinase Cdc2 MPF p33 protein kinase p33(CDK2) |
Images
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Fig1:
Western blot analysis of Cdk2 on different cell lysates using anti-Cdk2 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: F9 cell lysate Lane 3: PC-12 cell lysate Lane 4: Mouse thymus tissue lysate Lane 5: Human liver tissue lysate |
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Fig2:
Western blot analysis of Cdk2 on different lysates with Rabbit anti-Cdk2 antibody (R1309-3) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Cdk2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minute; ECL: merk 4-20% SDS-PAGE gel. R1309-3 was shown to specifically react with Cdk2 in Hela-si NT cells. Weakened band was observed when Hela-si Cdk2 sample was tested. Hela-si NT and Hela-si Cdk2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1309-3, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining Cdk2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cdk2 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with Cdk2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.