NF-kB p105 / p50 Rabbit Polyclonal Antibody
cat.: R1309-9
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 105/50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NFKB1 aa 320-366. |
| Positive control: | Hela cell lysates, HepG2 cell lysates, Raji cell lysates, HeLa, mouse stomach tissue, rat stomach tissue, human breast carcinoma tissue. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:100 1:200-1:1,000 |
| Uniprot #: | SwissProt: P19838 Human | P25799 Mouse | Q63369 Rat |
| Alternative names: | DKFZp686C01211 DNA binding factor KBF1 DNA binding factor KBF1 EBP1 DNA-binding factor KBF1 EBP 1 EBP-1 EBP1 KBF1 MGC54151 NF kappa B NF kappaB NF kappabeta NF kB1 NFkappaB NFKB 1 NFKB p105 NFKB p50 Nfkb1 NFKB1_HUMAN Nuclear factor kappa B DNA binding subunit Nuclear factor kappa-B, subunit 1 Nuclear factor NF kappa B p105 subunit Nuclear factor NF kappa B p50 subunit Nuclear factor NF-kappa-B p50 subunit Nuclear factor of kappa light chain gene enhancer in B cells 1 Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 p105 p50 p84/NF-kappa-B1 p98 Transcription factor NFKB2 NF-κB p105/p50 NF κB p105/p50 NF-κB p105/p50 |
Images
|
Fig1: Western blot analysis on Hela (1), HepG2 (2) and Raji (3) cell lysates using anti-NF-κB p105/p50 rabbit polyclonal antibodies. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling NF-kB p105 / p50 with Rabbit anti-NF-kB p105 / p50 antibody (R1309-9) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-kB p105 / p50 antibody (R1309-9) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-NF-kB p105 / p50 antibody (R1309-9) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1309-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-NF-kB p105 / p50 antibody (R1309-9) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1309-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p105/p50 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.