Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 174 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein with Mouse TOP2A aa 106-353 / 1,528. |
Positive control: | Jurkat, A431 |
Subcellular location: | Nucleus |
Recommended Dilutions:
WB IF-Cell |
1:1,000-1:2,000 1:200 |
Uniprot #: | SwissProt: Q01320 Mouse |
Alternative names: | alpha isozyme ATP hydrolyzing DNA topoisomerase II alfa DNA gyrase DNA topoisomerase (ATP hydrolyzing) DNA topoisomerase 2 alpha DNA topoisomerase 2-alpha DNA topoisomerase II 170 kD DNA topoisomerase II alpha isozyme DNA topoisomerase II DNA Topoisomerase2 TOP 2A TOP2 TOP2A TOP2A_HUMAN Topoisomerase DNA II alpha 170kDa TP2A |
Fig1: Western blot analysis on Jurkat cell lysates using anti-TOP2A rabbit polyclonal antibodies. | |
Fig2: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |