| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 35, 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Bag1 full sequence. |
| Positive control: | Hela, SW480, F9, HCT116, HT-29, HepG2, 293, 293T, A431 |
| Subcellular location: | Cytoplasm. Nucleus |
| Recommended Dilutions:
WB IF-Cell |
1:1000 1:200 |
| Uniprot #: | SwissProt: Q99933 Human |
| Alternative names: | Bag 1 BAG family molecular chaperone regulator 1 BAG-1 BAG1 BAG1_HUMAN BCL 2 Associated Athanogene 1 BCL 2 associated athanogene BCL 2 binding athanogene 1 Bcl-2-associated athanogene 1 BCL2 associated athanogene 1 BCL2 associated athanogene Glucocorticoid receptor-associated protein RAP46 HAP1 Haptoglobin RAP 46 RAP46 |
|
Fig1: Western blot analysis on different cell lysates using anti-Bag1 rabbit polyclonal antibodies. |
|
Fig2: ICC staining Bag1 in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
|
Fig3: ICC staining Bag1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |