TRAF6 Rabbit Polyclonal Antibody
cat.: R1311-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Chicken, Zebrafish |
| Applications: | IF-Cell, WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human TRAF6. |
| Positive control: | CTLL-2, EL4 , PC-12, L6, HeLa, hybrid fish (crucian-carp) heart tissue lysate. |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:100-1:200 |
| Uniprot #: | SwissProt: Q9Y4K3 Human | P70196 Mouse | B5DF45 Rat |
| Alternative names: | E3 ubiquitin-protein ligase TRAF6 Interleukin 1 signal transducer Interleukin-1 signal transducer MGC 3310 MGC:3310 MGC3310 OTTHUMP00000232772 OTTHUMP00000232773 RING finger protein 85 RNF 85 RNF85 TNF receptor associated factor 6 TNF receptor-associated factor 6 TNF receptor-associated factor 6, E3 ubiquitin protein ligase TRAF 6 Traf6 TRAF6_HUMAN |
Images
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Fig1:
Western blot analysis of TRAF6 on different lysates with Rabbit anti-TRAF6 antibody (R1311-2) at 1/1,000 dilution. Lane 1: CTLL-2 (Mouse T lymphocyte) cell lysate Lane 2: EL4 (Mouse lymphoma cell) cell lysate Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 4: L6 (Rat skeletal muscle myoblast) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 120 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1311-2, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59.6 kDa Observed band size: 60 kDa |
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Fig2:
Western blot analysis of TRAF6 on different lysates with Rabbit anti-TRAF6 antibody (R1311-2) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1311-2, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59.6 kDa Observed band size: 60 kDa |
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Fig3: ICC staining TRAF6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Western blot analysis of TRAF6 on hybrid fish (crucian-carp) heart tissue lysate using anti-TRAF6 antibody at 1/500 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.