BMPR1B Rabbit Polyclonal Antibody
cat.: R1311-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BMPR1B aa 14-126. |
| Positive control: | HepG2, SHG-44, human ovarian carcinoma tissue, mouse brain tissue. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1000 1:100-1:500 1:200 |
| Uniprot #: | SwissProt: O00238 Human | P36898 Mouse |
| Alternative names: | Activin receptor like kinase 6 Acvrlk6 ALK 6 ALK6 alk6tr BMP type-1B receptor BMPR IB BMPR-1B Bmpr1b BMPRIB BMR1B_HUMAN Bone morphogenetic protein receptor type 1B Bone morphogenetic protein receptor type IB Bone morphogenetic protein receptor type-1B BR 1b BR1b CDw 293 CDw293 CDw293 antigen CFK 43a CFK43a Serine/threonine receptor kinase zALK 6 zALK6 |
Images
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Fig1: Western blot analysis on SHG-44 cell lysates using anti- BMPR1B rabbit polyclonal antibodies. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling BMPR1B with Rabbit anti-BMPR1B antibody (R1311-4) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-BMPR1B antibody (R1311-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using anti- BMPR1B rabbit polyclonal antibody. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-BMPR1B antibody (R1311-4) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1311-4) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.