Geminin Rabbit Polyclonal Antibody
cat.: R1311-8
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein with Mouse Geminin aa 70-206 / 206. |
| Positive control: | F9, D3, human testis tissue. |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
IHC-P IF-Cell |
1:100-1:200 1:200 |
| Uniprot #: | SwissProt: O88513 Mouse |
| Alternative names: | DNA replication inhibitor Gem GEMI_HUMAN Geminin Geminin DNA replication inhibitor GMNN OTTHUMP00000039393 RP3 369A17.3 |
Images
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Fig1: ICC staining Geminin in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
|
Fig2: ICC staining Geminin in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Geminin antibody (R1311-8) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1311-8) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.