GSK3 alpha Rabbit Polyclonal Antibody
cat.: R1312-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GSK3 alpha aa 434-483 / 483. |
| Positive control: | Hela, HepG2, PC-3M, Jurkat, human brain |
| Subcellular location: | Cytoplasm, Nucleus, Apical dendrite, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell |
1:1000 1:100 |
| Uniprot #: | SwissProt: P49840 Human |
| Alternative names: | DKFZp686D0638 Glycogen synthase kinase 3 alpha Glycogen synthase kinase-3 alpha GSK 3 alpha GSK 3A GSK-3 alpha Gsk3a GSK3A_HUMAN GSK3alpha Serine/threonine protein kinase GSK3A |
Images
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Fig1: Western blot analysis on different cell lysates using anti-GSK3 alpha rabbit polyclonal antibodies. |
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Fig2:
Western blot analysis of GSK3 alpha on different lysates with Rabbit anti-GSK3 alpha antibody (R1312-1) at 1/1,000 dilution. Lane 1: A549-WT cell lysate (10 µg/Lane) Lane 2: A549-KD GSK3 alpha cell lysate (10 µg/Lane) Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1312-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining GSK3 alpha in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.