MKRN2 Rabbit Polyclonal Antibody
cat.: R1312-14
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human MRKN2 aa 281-330 / 416. |
| Positive control: | HepG2, Jurkat, 293T, mouse liver tissue, Hela, human liver tisse, mouse thymus tissue, human colorectal carcinomas tissue |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:200-1:500 1:50 1:200-1:500 |
| Uniprot #: | SwissProt: Q9H000 Human | Q9ERV1 Mouse |
| Alternative names: | HSPC070 Makorin 2 Makorin ring finger protein 2 Makorin2 MKRN 2 RING finger protein 62 RNF 62 RNF62 |
Images
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Fig1: Western blot analysis on cell lysates using anti-MKRN2 rabbit polyclonal antibodies. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling MKRN2 with Rabbit anti-MKRN2 antibody (R1312-14) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MKRN2 antibody (R1312-14) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of Hela cells labeling MKRN2 with Rabbit anti-MKRN2 antibody (R1312-14) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MKRN2 antibody (R1312-14) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MKRN2 antibody (R1312-14) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1312-14) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-MKRN2 antibody (R1312-14) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1312-14) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colorectal carcinomas tissue using anti- MKRN2 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.