GFP Rabbit Polyclonal Antibody
cat.: R1312-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of Aequorea victoria GFP. |
| Positive control: | GFP protein |
| Recommended Dilutions:
WB ELISA IP |
1:10,000 1:10,000 2-5 µg/ml. |
| Uniprot #: | SwissProt: P42212 Aequorea victoria |
| Alternative names: | GFP Green fluorescent protein yfp |
Images
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Fig1: Western blot analysis on GFP protein using rabbit polyclonal antibody. |
|
Fig2:
GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with R1312-2 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: GFP Tag fusion protein lysate (input). Lane 2: Rabbit IgG instead of R1312-2 in GFP Tag fusion protein lysate. Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate. Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate. Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate. Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate. Lane 7: R1312-2 IP in GFP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.