GFP Rabbit Polyclonal Antibody
cat.: R1312-2
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Species independent
Applications: WB, ELISA, IP
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Recombinant full length protein of Aequorea victoria GFP.
Positive control: GFP protein
Recommended Dilutions:
  WB
  ELISA
  IP

1:10,000
1:10,000
2-5 µg/ml.
Uniprot #: SwissProt: P42212 Aequorea victoria
Alternative names: GFP Green fluorescent protein yfp
Images
R1312-2_1.jpg Fig1: Western blot analysis on GFP protein using rabbit polyclonal antibody.
R1312-2_2.jpg Fig2: GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with R1312-2 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.

Lane 1: GFP Tag fusion protein lysate (input).
Lane 2: Rabbit IgG instead of R1312-2 in GFP Tag fusion protein lysate.
Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate.
Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate.
Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate.
Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate.
Lane 7: R1312-2 IP in GFP Tag fusion protein lysate.

Blocking/Dilution buffer: 5% NFDM/TBST
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.