DPY30 Rabbit Polyclonal Antibody
cat.: R1312-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11kDa |
| Isotype: | IgG |
| Immunogen: | Full length protein. |
| Positive control: | NCCIT , F9, D3, human lung carcinoma tissue, mouse brain tissue. |
| Subcellular location: | Nucleus. Golgi apparatus |
| Recommended Dilutions:
IHC-P IF-Cell |
1:100-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: Q9C005 Human | Q99LT0 Mouse |
| Alternative names: | Dpy-30-like protein Dpy-30L DPY30 DPY30_HUMAN HDPY-30 Protein dpy-30 homolog Saf19 |
Images
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Fig1:
Immunocytochemistry analysis of NCCIT cells labeling DPY30 with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig2:
Immunocytochemistry analysis of F9 cells labeling DPY30 with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: ICC staining DPY30 in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- DPY30 rabbit polyclonal antibody. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DPY30 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.