Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IHC-P, IF-Tissue |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage buffer: | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 68 kDa |
Isotype: | IgG |
Immunogen: | KLH conjugated synthetic peptide derived from human Nrf2: 401-500/605 |
Subcellular location: | Cytoplasm, Nucleus |
Recommended Dilutions:
WB IHC-P IF-tissue |
1:100-1000 1:100-500 1:100-500 |
Uniprot #: | SwissProt: Q16236 Human | Q60795 Mouse |
Alternative names: | erythroid derived 2 HEBP1 like 2 NF E2 related factor 2 NF-E2-related factor 2 NF2L2_HUMAN NFE2 related factor 2 NFE2-related factor 2 Nfe2l2 Nrf 2 NRF2 Nuclear factor (erythroid derived 2) like 2 Nuclear factor nuclear factor erythroid 2 like 2 Nuclear factor erythroid 2 related factor 2 Nuclear factor erythroid 2-related factor 2 Nuclear factor erythroid derived 2 like 2 |
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Fig1: Hep-G2 (H) cells were treated with or without MG-132 (10 μM) for 10h, 25 μg total protein per lane of cell lysates (see on figure) probed with Nrf2 polyclonal antibody, unconjugated (bs-1074R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |
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Fig2: Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig3: Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig4: Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig5: Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig6: Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig7: Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig8: Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig9: Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig10: Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig11: Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
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Fig12: Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |