Nrf2 Rabbit Polyclonal Antibody
cat.: R1312-8
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue, FC, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 68 kDa
Isotype: IgG
Immunogen: KLH conjugated synthetic peptide derived from human Nrf2
Subcellular location: Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
  FC
  IF-Cell
1:1000-5000
1:200-1000
1:200-1000
1μg/Test
1:100-500
Uniprot #: SwissProt: Q16236 Human | Q60795 Mouse
Alternative names: erythroid derived 2 HEBP1 like 2 NF E2 related factor 2 NF-E2-related factor 2 NF2L2_HUMAN NFE2 related factor 2 NFE2-related factor 2 Nfe2l2 Nrf 2 NRF2 Nuclear factor (erythroid derived 2) like 2 Nuclear factor nuclear factor erythroid 2 like 2 Nuclear factor erythroid 2 related factor 2 Nuclear factor erythroid 2-related factor 2 Nuclear factor erythroid derived 2 like 2
Images
R1312-8_1.jpg Fig1: Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_2.jpg Fig2: Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_3.jpg Fig3: Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_4.jpg Fig4: Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_5.jpg Fig5: Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_6.jpg Fig6: Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_7.jpg Fig7: Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_8.jpg Fig8: Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_9.jpg Fig9: Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_10.jpg Fig10: Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_11.jpg Fig11: Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
R1312-8_12.jpg Fig12: Hela (H) cells were treated with or without MG-132 (10 μM) for 10h, 25 μg total protein per lane of cell lysates (see on figure) probed with Nrf2 polyclonal antibody, unconjugated (R1312-8) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
R1312-8_13.jpg Fig13: Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
R1312-8_14.jpg Fig14: Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
R1312-8_15.jpg Fig15: The HeLa（treated with MG-132 (10 μM,10h) ） (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Nrf2 antibody (R1312-8,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
R1312-8_16.jpg Fig16: 4% Paraformaldehyde-fixed Hela（treated with MG-132 (10 μM,10h) ）(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Nrf2) polyclonal Antibody, unconjugated (R1312-8) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.