Y14 Rabbit Polyclonal Antibody
cat.: R1401-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Y14 aa 125-174 / 174. |
| Positive control: | SH-SY5Y, MCF-7, RWPE-1, SW620, mouse kidney tissue, mouse heart tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y5S9 Human | Q9CWZ3 Mouse | Q27W01 Rat |
| Alternative names: | Binder of OVCA1 1 Binder of OVCA1-1 BOV 1 BOV 1A BOV 1B BOV 1C BOV-1 BOV1 BOV1A BOV1B BOV1C HSPC 114 HSPC114 MDS 014 MDS014 RBM 8 RBM 8A RBM 8B RBM8 rbm8a RBM8A_HUMAN RBM8B Ribonucleoprotein RBM 8 Ribonucleoprotein RBM 8A Ribonucleoprotein RBM8 Ribonucleoprotein RBM8A RNA binding motif protein 8 RNA binding motif protein 8A RNA binding motif protein 8B RNA binding protein 8A RNA binding protein Y14 RNA-binding motif protein 8A RNA-binding protein 8A RNA-binding protein Y14 ZNRP ZRNP 1 ZRNP1 |
Images
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Fig1: Western blot analysis of Y14 on SH-SY5Y cell lysates with Rabbit anti-Y14 antibody (R1401-10) at 1/500 dilution. |
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Fig2: ICC staining Y14 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
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Fig3:
Immunocytochemistry analysis of MCF-7 cells labeling Y14 with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of RWPE-1 cells labeling Y14 with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of SW620 cells labeling Y14 with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Y14 rabbit polyclonal antibody. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- Y14 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.