TCF7L2/TCF4 Rabbit Polyclonal Antibody
cat.: R1401-11
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 68 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminalHuman TCF4 aa 1-50 / 619.
Positive control: Hela, HepG2, HCT116, SW480, rat kidney tissue, human tonsil tissue, human colon cancer tissue, human breast cancer tissue.
Subcellular location: Nucleus
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-1:2,000
1:500-1:2,000
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: Q9NQB0 Human | Q924A0 Mouse | D3Z9D1 Rat
Alternative names: bHLHb19 Class B basic helix-loop-helix protein 19 E2 2 FECD3 Immunoglobulin transcription factor 2 ITF 2 ITF-2 ITF2 ITF2_HUMAN MGC149723 MGC149724 PTHS SEF 2 SEF-2 SEF2 1 SEF2 1A SEF2 1B SEF2 1D SEF2 SL3 3 enhancer factor 2 SL3-3 enhancer factor 2 TCF 4 TCF-4 TCF4 Transcription factor 4 Transcription factor 4, isoform C Transcription factor 4, isoform D Transcription factor 4, isoform E Transcription factor 4, isoform L Transcription factor 4, isoform M Transcription factor 4, isoform R
Images
R1401-11_1.jpg Fig1: Western blot analysis of TCF4 on different cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1401-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. (with different isoforms)
R1401-11_2.jpg Fig2: ICC staining of TCF4 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1401-11, 1/500) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
R1401-11_3.jpg Fig3: ICC staining of TCF4 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1401-11, 1/500) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
R1401-11_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney using anti-TCF4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1401-11, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1401-11_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil using anti-TCF4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1401-11, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1401-11_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TCF4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1401-11, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1401-11_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TCF4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1401-11, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1401-11_8.jpg Fig8: Flow cytometric analysis of HepG2 cells with TCF4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.