AU1 tag Rabbit Polyclonal Antibody
cat.: R1401-20
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | This antibody is produced by immunizing rabbits with a synthetic peptide DTYRYI (KLH-coupled). |
| Positive control: | N-terminal AU1 tagged Recombinant protein. |
| Recommended Dilutions:
WB ELISA |
1:1,000-1:5,000 1:10,000 |
| Alternative names: | AU1 epitope tag DTYRYI epitope tag DTYRYI tag |
Images
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Fig1:
Western blot analysis of AU1 tag on N-terminal AU1 tagged Recombinant protein with Rabbit anti-AU1 tag antibody (R1401-20) at 1/1,000 dilution. Lysates/proteins at 100 ng/Lane. Exposure time: 59 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1401-20, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.