Beta III Tubulin Rabbit Polyclonal Antibody
cat.: R1401-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human Beta III Tubulin aa 401-450 / 450. |
| Positive control: | SH-SY5Y, Neuro-2a, mouse brain, rat brain |
| Subcellular location: | Cytoplasm , cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell |
1:2000-1:5000 1:100-1:200 |
| Uniprot #: | SwissProt: Q13509 Human |
| Alternative names: | beta 3 tubulin beta 4 beta-4 CDCBM CDCBM1 CFEOM3 CFEOM3A FEOM3 M(beta)3 M(beta)6 MC1R Neuron specific beta III Tubulin Neuron-specific class III beta-tubulin QccE-11995 QccE-15186 TBB3_HUMAN Tubb 3 TUBB3 TUBB4 Tubulin beta 3 Tubulin beta 3 chain Tubulin beta 4 Tubulin beta III Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-III tuj 1 tuj1 |
Images
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Fig1:
Western blot analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody (R1401-3) at 1/5,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Cell Lysates at 15 µg/Lane,tissueell Lysates at 30 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1401-3, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 50 kDa |
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Fig2: ICC staining beta III tubulin in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.