NSE Rabbit Polyclonal Antibody
cat.: R1401-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish, Hybrid fish (crucian-carp) |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human NSE. |
| Positive control: | Hela, NIH/3T3, F9, human brain, mouse brain, A172, SHG-44, N2A hybrid fish (crucian-carp) brain tissue lysate. |
| Subcellular location: | Cytoplasm, cell membrane |
| Recommended Dilutions:
WB IF-Cell |
1:2000-1:5,000 1:100 |
| Uniprot #: | SwissProt: P09104 Human |
| Alternative names: | 2 phospho D glycerate hydrolyase 2-phospho-D-glycerate hydro-lyase Eno 2 ENO2 ENOG ENOG_HUMAN Enolase 2 (gamma, neuronal) Enolase 2 Enolase 2 gamma neuronal Enolase2 Epididymis secretory protein Li 279 Gamma enolase Gamma-enolase HEL S 279 Neural enolase Neuron specific enolase Neuron specific gamma enolase Neuron-specific enolase neuronal enriched enolase Neurone specific enolase NSE |
Images
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Fig1:
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (R1401-6) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NSE KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1401-6) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis on different cell lysates using anti-NSE rabbit polyclonal antibodies. |
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Fig3: Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysate using anti-NSE antibody at 1/500 dilution. |
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Fig4: ICC staining NSE in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.