Glucose Transporter GLUT4 Rabbit Polyclonal Antibody
cat.: R1402-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Glucose Transporter GLUT4 aa 460-509 / 509. |
| Positive control: | Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, mouse heart tissue. |
| Subcellular location: | Cell membrane, Endomembrane system, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P14672 Human | P14142 Mouse | P19357 Rat |
| Alternative names: | Glucose transporter GLUT 4 Glucose transporter type 4 Glucose transporter type 4 insulin responsive GLUT 4 GLUT-4 GLUT4 GTR4_HUMAN Insulin responsive glucose transporter type 4 insulin-responsive kug SLC 2A4 SLC2A4 solute carrier family 2 (facilitated glucose transporter) member 4 Solute carrier family 2 member 4 Solute carrier family 2, facilitated glucose transporter member 4 |
Images
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Fig1:
Western blot analysis of Glucose Transporter GLUT4 on different lysates with Rabbit anti-Glucose Transporter GLUT4 antibody (R1402-3) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate (no heat) Lane 2: Mouse skeletal muscle tissue lysate (no heat) Lane 3: Rat skeletal muscle tissue lysate (no heat) Lane 4: Rat kidney tissue lysate (low expression) (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1402-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining GLUT4 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-GLUT4 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.