AU5 tag Rabbit Polyclonal Antibody
cat.: R1402-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein fused with AU5 Tag. |
| Positive control: | AU5 tagged fusion protein |
| Recommended Dilutions:
WB ELISA |
1:5,000 1:10,000 |
| Alternative names: | AU5 epitope tag TDFYLK epitope tag TDFYLK tag |
Images
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Fig1:
Western blot analysis of AU5 tag on AU5 tagged fusion protein with Rabbit anti-AU5 tag antibody (R1402-4) at 1/1,000 dilution. Lane 1: AU5 tagged fusion protein, 1,000ng/Lane Lane 2: AU5 tagged fusion protein, 200ng/Lane Lane 3: AU5 tagged fusion protein, 50ng/Lane Exposure time: 51 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1402-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.