CRISPR-Cas9 Rabbit Polyclonal Antibody
cat.: R1403-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Staphylococcus aureus |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within StreptococcusThermophilus CRISPR-Cas9 aa 374-415/1388. |
| Positive control: | Cas9 transfected 293 cells |
| Recommended Dilutions:
WB ELISA |
1:2,000-1:5,000 1:10,000 |
| Uniprot #: | SwissProt: Q03JI6 StreptococcusThermophilus |
| Alternative names: | Cas9 CRISPR-associated endonuclease Cas9/Csn1 CRISPR-Cas9/Csn1 csn1 SpyCas9 |
Images
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Fig1:
Western blot analysis of CRISPR-Cas9 on different lysates with Rabbit anti-CRISPR-Cas9 antibody (R1403-1) at 1/1,000 dilution. Lane 1: 293-WT cell lysate Lane 2: 293T-OE cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 180 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1403-1, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Observed band size: 158kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.