CUGBP Elav-like family member 2 Rabbit Polyclonal Antibody
cat.: R1403-3
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to Human CUGBP Elav-like family member 2 aa450-508 / 508.
Positive control: PC-3M cell lysate, Jurkat cell lysate, THP-1 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat cerebellum tissue lysate, Hela, MCF-7, SHG-44.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
1:1,000
1:50-1:200
Uniprot #: SwissProt: O95319 Human | Q9Z0H4 Mouse | Q792H5 Rat
Alternative names: Bruno-like protein 3 BRUNOL3 Cardiac elav type RNA binding protein CELF-2 celf2 CELF2_HUMAN CUG BP2 CUG triplet repeat RNA-binding protein 2 CUG-BP- and ETR-3-like factor 2 CUG-BP2 CUGBP Elav like family member 2 CUGBP Elav-like family member 2 CUGBP2 dJ323N1.1 elav type RNA binding protein ELAV-type RNA-binding protein 3 ETR 3 ETR-3 ETR3 HGNC:2550 hNAPOR NAPOR 2 NAPOR Neuroblastoma apoptosis-related RNA-binding protein Neuroplastoma apoptosis related RNA binding protein 3 RNA binding protein BRUNOL3 RNA-binding protein BRUNOL-3
Images
R1403-3_1.jpg Fig1: Western blot analysis of CUGBP Elav-like family member 2 on different lysates with Rabbit anti-CUGBP Elav-like family member 2 antibody (R1403-3) at 1/1,000 dilution.

Lane 1: PC-3M cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: THP-1 cell lysate (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (30 µg/Lane)
Lane 5: Rat brain tissue lysate (30 µg/Lane)
Lane 6: Rat cerebellum tissue lysate (30 µg/Lane)

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1403-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
R1403-3_2.jpg Fig2: ICC staining CELF2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1403-3_3.jpg Fig3: ICC staining CELF2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1403-3_4.jpg Fig4: ICC staining CELF2 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.