ATG12 Rabbit Polyclonal Antibody
cat.: R1404-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ATG12 aa 96-138. |
| Positive control: | Rat kidney lysates, rat pancreas tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: O94817 Human | Q9CQY1 Mouse | Q2TBJ5 Rat |
| Alternative names: | APG12-like APG12L ATG12 ATG12 autophagy related 12 homolog (S. cerevisiae) ATG12 autophagy related 12 homolog ATG12_HUMAN Autophagy 12 Autophagy-related protein 12 FBR93 HAPG12 Ubiquitin-like protein ATG12 |
Images
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Fig1: Western blot analysis on rat kidney lysates using anti-ATG12 rabbit polyclonal antibodies. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-ATG12 antibody (R1404-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1404-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ATG12 antibody (R1404-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1404-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.