UGP2 Rabbit Polyclonal Antibody
cat.: R1404-5
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human UGP2 aa 60-120. |
| Positive control: | Human liver tissue lysate, Rat liver tissue lysate, Mouse liver tissue lysate |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q16851 Human |
| Alternative names: | pHC379 UDP glucose diphosphorylase UDP glucose pyrophosphorylase 1 UDP glucose pyrophosphorylase 2 UDP glucose pyrophosphorylase UDP-glucose pyrophosphorylase UDPG UDPGP 2 UDPGP UDPGP2 UGP 1 UGP 2 UGP1 Ugp2 UGPA_HUMAN UGPase 2 UGPase UGPP 1 UGPP 2 UGPP1 UGPP2 Uridyl diphosphate glucose pyrophosphorylase 1 Uridyl diphosphate glucose pyrophosphorylase 2 UTP glucose 1 phosphate uridyltransferase UTP glucose 1 phosphate uridylyltransferase 2 UTP glucose 1 phosphate uridylyltransferase UTP--glucose-1-phosphate uridylyltransferase |
Images
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Fig1:
Western blot analysis of UGP2 on different lysates with Rabbit anti-UGP2 antibody (R1404-5) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Mouse liver tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1404-5, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature |
|
Fig2:
Western blot analysis of UGP2 on different lysates with Rabbit anti-UGP2 antibody (R1404-5) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-UGP2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 57/56 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1404-5) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.