Axl Rabbit Polyclonal Antibody
cat.: R1406-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AXL aa 1-451. |
| Positive control: | HeLa cell lysate, NCI-H1299 cell lysate, C2C12 cell lysate, human liver tissue, NCI-H1299. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:2,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P30530 Human | Q00993 Mouse |
| Alternative names: | Adhesion related kinase AI323647 Ark Axl AXL oncogene AXL receptor tyrosine kinase AXL transforming gene AXL transforming sequence/gene EC 2.7.10.1 JTK11 Oncogene AXL Tyro7 Tyrosine protein kinase receptor UFO Tyrosine-protein kinase receptor UFO UFO UFO_HUMAN |
Images
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Fig1:
Western blot analysis of Axl on different lysates with Rabbit anti-Axl antibody (R1406-3) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: NCI-H1299 cell lysate Lane 3: Jurkat cell lysate (negative) Lane 4: C2C12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 140/150 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1406-3) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of AXL on human AXL recombinant protein using anti-AXL antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Axl antibody (R1406-3) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1406-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of NCI-H1299 cells labeling Axl with Rabbit anti-Axl antibody (R1406-3) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Axl antibody (R1406-3) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of NCI-H1299 cells labeling Axl. Cells were fixed and permeabilized. Then stained with the primary antibody (R1406-3, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.