KLHL15 Rabbit Polyclonal Antibody
cat.: R1407-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human KLHL15 aa 555-604 / 604. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, Hela, PANC-1. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100-1:200 |
| Uniprot #: | SwissProt: Q96M94 Human |
| Alternative names: | Kelch like 15 (Drosophila) Kelch like 15 Kelch-like protein 15 KIAA1677 KLH15_HUMAN KLHL15 MGC126148 MGC126149 OTTHUMP00000023075 |
Images
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Fig1:
Western blot analysis of KLHL15 on different lysates with Rabbit anti-KLHL15 antibody (R1407-2) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 75 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1407-2) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining KLHL15 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining KLHL15 in PANC-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.