ALDH2 Rabbit Polyclonal Antibody
cat.: R1407-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ALDH2 aa 18-80. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, THP-1 cell lysate, human liver tissue lysate, rat lung tissue lysate, Hela, HepG2. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P05091 Human | P47738 Mouse | P11884 Rat |
| Alternative names: | Acetaldehyde dehydrogenase 2 Aldehyde dehydrogenase 2 family (mitochondrial) Aldehyde dehydrogenase 2 family Aldehyde dehydrogenase mitochondrial Aldehyde dehydrogenase, mitochondrial ALDH 2 ALDH class 2 ALDH E2 ALDH-E2 Aldh2 ALDH2_HUMAN ALDHI ALDM Liver mitochondrial ALDH MGC1806 Mitochondrial aldehyde dehydrogenase 2 MS767 Nucleus encoded mitochondrial aldehyde dehydrogenase 2 |
Images
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Fig1:
Western blot analysis of ALDH2 on different lysates with Rabbit anti-ALDH2 antibody (R1407-3) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: SK-Br-3 cell lysate (15 µg/Lane) Lane 4: THP-1 cell lysate (15 µg/Lane) Lane 5: Human liver tissue lysate (30 µg/Lane) Lane 6: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1407-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining ALDH2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
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Fig3: ICC staining ALDH2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.