ASH2L Rabbit Polyclonal Antibody
cat.: R1408-1
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 59/57/60
Isotype: IgG
Immunogen: Synthetic peptide within human ASH2L aa 575-628.
Positive control: Hela cell lysates, PC-12 cell lysates, mouse colon tissue, human breast carcinoma tissue, human colon tissue, Siha.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: Q9UBL3 Human | Q91X20 Mouse
Alternative names: ASH 2 Ash2 (absent small or homeotic) like Ash2 (absent, small, or homeotic) like (Drosophila) ASH2 ASH2 LIKE ASH2 like protein ASH2-like protein Ash2l ASH2L_HUMAN ASH2L1 ASH2L2 Bre 2 Bre2 Discs 2-like Drosophila absent, small, or homeotic Drosophila ASH2-like Set1/Ash2 histone methyltransferase complex subunit ASH2
Images
R1408-1_1.jpg Fig1: Western blot analysis of ASH2L on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1408-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
R1408-1_2.jpg Fig2: Western blot analysis of ASH2L on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1408-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
R1408-1_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ASH2L antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1408-1, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1408-1_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ASH2L antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1408-1, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1408-1_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ASH2L antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1408-1, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1408-1_6.jpg Fig6: Flow cytometric analysis of ASH2L was done on Siha cells. The cells were fixed, permeabilized and stained with the primary antibody (R1408-1, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.