STAT1 Rabbit Polyclonal Antibody
cat.: R1408-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human STAT1 aa 87-262. |
| Positive control: | Hela, HT-29 cell lysate |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P42224 Human | P42225 Mouse Entrez Gene: 25124 Rat |
| Alternative names: | Signal transducer and activator of transcription 1 91kD CANDF7 DKFZp686B04100 IMD31A IMD31B IMD31C ISGF 3 ISGF-3 OTTHUMP00000163552 OTTHUMP00000165046 OTTHUMP00000165047 OTTHUMP00000205845 Signal transducer and activator of transcription 1 91kDa Signal transducer and activator of transcription 1 Signal transducer and activator of transcription 1, 91kD Signal transducer and activator of transcription 1-alpha/beta STAT 1 Stat1 STAT1_HUMAN STAT91 Transcription factor ISGF 3 components p91 p84 Transcription factor ISGF-3 components p91/p84 |
Images
|
Fig1: ICC staining STAT1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2:
Western blot analysis of STAT1 on HT-29 cell lysate with Rabbit anti-STAT1 antibody (R1408-2) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1408-2, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 87 kDa Observed band size: 87 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.