Lamin B2 Rabbit Polyclonal Antibody
cat.: R1408-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within huamn Lamin B2 aa 1-49/620. |
| Positive control: | A431 cell lysate, HepG2 cell lysate, K-562 cell lysate, A549 cell lysate, Jurkat cell lysate, Raji cell lysate, HCT116 cell lysate. |
| Subcellular location: | Nucleus lamina. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q03252 Human |
| Alternative names: | Lamin-B2 Lamin B2 LMN 2 LMN B2 LMN2 LMNB 2 LMNB2 LMNB2_HUMAN MGC2721 RGD1563803 |
Images
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Fig1:
Western blot analysis of Lamin B2 on different lysates with Rabbit anti-Lamin B2 antibody (R1408-4) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: HepG2 cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: Jurkat cell lysate Lane 6: Raji cell lysate Lane 7: HCT116 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1408-4) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.