Psg16 Rabbit Polyclonal Antibody
cat.: R1408-5
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Psg16 aa 346-395 / 395. |
| Positive control: | Mouse brain, D3 |
| Subcellular location: | Cell membrane, Cell projection |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100-1:200 |
| Uniprot #: | SwissProt: Q8K0U8 Mouse |
| Alternative names: | Pregnancy specific glycoprotein 16, Pregnancy-specific glycoprotein 16 |
Images
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Fig1: ICC staining Psg16 in mouse embryonic stem cells D3 (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
|
Fig2:
Western blot analysis of Psg16 on different lysates with Rabbit anti-Psg16 antibody (R1408-5) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1408-5, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 45 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.