ERp72 Rabbit Polyclonal Antibody
cat.: R1409-1
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminalhuman ERP72.
Positive control: HeLa cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, PANC-1 cell lysate, PC-12 cell lysate, human liver tissue lysate, mouse liver tissue lysate, A549, PC-12, human lung cancer tissue.
Subcellular location: Endoplasmic reticulum lumen, Melanosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:5,000
1:500
1:200
Uniprot #: SwissProt: P13667 Human | P08003 Mouse | P38659 Rat
Alternative names: Calcium binding protein intestinal related Endoplasmic reticulum resident protein 70 Endoplasmic reticulum resident protein 72 EPR70 EPR72 ER protein 70 ER protein 72 ERP 70 ERp 72 ERp-72 ERp70 ERp72 PDIA 4 Pdia4 PDIA4_HUMAN PDIR Protein disulfide isomerase A4 Protein disulfide isomerase associated 4 Protein disulfide isomerase family A member 4 protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) Protein disulfide isomerase related protein Protein disulfide-isomerase A4 Protein ERp 72 Protein ERp72
Images
R1409-1_1.jpg Fig1: Western blot analysis of ERp72 on different lysates with Rabbit anti-ERp72 antibody (R1409-1) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SK-Br-3 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: PANC-1 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)
Lane 7: Mouse liver tissue lysate (40 µg/Lane)

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1409-1) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
R1409-1_2.jpg Fig2: ICC staining ERp72 in A549 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1409-1) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
R1409-1_3.jpg Fig3: ICC staining ERp72 in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1409-1) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
R1409-1_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (R1409-1) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.