Gamma tubulin Rabbit Polyclonal Antibody
cat.: R1411-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Gamma tubulin. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human heart tissue lysate. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1: 1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P23258 Human | P83887 Mouse | P83888 Rat |
| Alternative names: | Gamma 1 tubulin Gamma 2 tubulin Gamma Tubulin 1 Gamma Tubulin 2 Gamma tubulin complex component 1 Gamma-2-tubulin GCP 1 GCP-1 GCP1 MGC131994 TBG2_HUMAN TUBG TUBG1 TUBG2 TUBGCP1 Tubulin gamma 1 chain Tubulin gamma 2 chain Tubulin gamma complex-associated protein 1 Tubulin gamma-2 chain tubulin, gamma 1 tubulin, gamma 2 tubulin, gamma polypeptide Xgam |
Images
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Fig1:
Western blot analysis of Gamma tubulin on different lysates with Rabbit anti-Gamma tubulin antibody (R1411-2) at 1/1,000 dilution. Lane 1: A431 (Human epidermal cancer cells) cell lysate Lane 2: C2C12(Mouse myoblast) cell lysate Lane 3: PC-12(Rat adrenal medulla pheochromocytoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20s seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1411-2, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 51 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.