Gamma tubulin Rabbit Polyclonal Antibody
cat.: R1411-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Gamma tubulin. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human heart tissue lysate. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1: 1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P23258 Human | P83887 Mouse | P83888 Rat |
| Alternative names: | Gamma 1 tubulin Gamma 2 tubulin Gamma Tubulin 1 Gamma Tubulin 2 Gamma tubulin complex component 1 Gamma-2-tubulin GCP 1 GCP-1 GCP1 MGC131994 TBG2_HUMAN TUBG TUBG1 TUBG2 TUBGCP1 Tubulin gamma 1 chain Tubulin gamma 2 chain Tubulin gamma complex-associated protein 1 Tubulin gamma-2 chain tubulin, gamma 1 tubulin, gamma 2 tubulin, gamma polypeptide Xgam |
Images
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Fig1:
Western blot analysis of Gamma tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1411-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Human heart tissue lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.