Eftud2 Rabbit Polyclonal Antibody
cat.: R1411-3
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 109 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Eftud2 aa 1-50 / 972.
Positive control: PC-12 cell lysate, Jurkat cell lysate, B16F2, Hela, SW480, human colon cancer tissue, mouse colon tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q15029 Human | O08810 Mouse
Alternative names: 116 kDa 116 kDa U5 small nuclear ribonucleoprotein component EFTUD2 Elongation factor Tu GTP binding domain containing 2 Elongation factor Tu GTP-binding domain-containing protein 2 hSNU114 MFDGA MFDM SNRNP116 Snrp116 Snu114 SNU114 homolog U5 116KD U5 small nuclear ribonucleoprotein component U5 snRNP specific protein, 116 kD U5 snRNP specific protein, 116 kDa U5 snRNP-specific protein U5-116 kDa U5-116KD U5S1_HUMAN
Images
R1411-3_1.jpg Fig1: Western blot analysis of Eftud2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-12 cell lysate, untreated
Lane 2: Jurkat cell lysate, untreated
R1411-3_2.jpg Fig2: ICC staining Eftud2 in B16F2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1411-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
R1411-3_3.jpg Fig3: ICC staining Eftud2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1411-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
R1411-3_4.jpg Fig4: ICC staining Eftud2 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1411-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
R1411-3_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Eftud2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (R1411-3) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
R1411-3_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Eftud2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (R1411-3) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
R1411-3_7.jpg Fig7: Flow cytometric analysis of Eftud2 was done on Hela cells. The cells were fixed, permeabilized and stained with Eftud2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.