HADH Rabbit Polyclonal Antibody
cat.: R1411-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human HADH aa 275-314 / 314. |
| Positive control: | MCF-7 cell lysate, human kidney tissue lysate, human colon carcinoma tissue, hela. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:100 1:50 |
| Uniprot #: | SwissProt: Q16836 Human |
| Alternative names: | 3 ketoacyl Coenzyme A (CoA) thiolase alpha subunit 3 oxoacyl CoA thiolase 78 kDa gastrin binding protein 78 kDa gastrin-binding protein ECHA ECHA_HUMAN GBP HADH HADHA Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit LCEH LCHAD Long chain 3-hydroxyacyl-CoA dehydrogenase Mitochondrial long chain 2 enoyl Coenzyme A (CoA) hydratase alpha subunit Mitochondrial long chain L 3 hydroxyacyl Coenzyme A dehydrogenase alpha subunit Mitochondrial trifunctional enzyme alpha subunit Mitochondrial trifunctional protein alpha subunit MTPA Thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit TP ALPHA TP-alpha Trifunctional enzyme subunit alpha mitochondrial precursor |
Images
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Fig1: Western blot analysis on cell lysates using anti- HADH rabbit polyclonal antibodies. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-HADH antibody (R1411-4) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1411-4) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of Hela cells labeling HADH with Rabbit anti-HADH antibody (R1411-4) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HADH antibody (R1411-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L was used as the secondary antibody. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.