HADH Rabbit Polyclonal Antibody
cat.: R1411-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human HADH aa 275-314 / 314. |
| Positive control: | HepG2 cell lysate, MCF7 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue, HeLa, HepG2, MCF7. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:5,000 1:100 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q16836 Human | Q61425 Mouse | Q9WVK7 Rat |
| Alternative names: | 3 ketoacyl Coenzyme A (CoA) thiolase alpha subunit 3 oxoacyl CoA thiolase 78 kDa gastrin binding protein 78 kDa gastrin-binding protein ECHA ECHA_HUMAN GBP HADH HADHA Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit LCEH LCHAD Long chain 3-hydroxyacyl-CoA dehydrogenase Mitochondrial long chain 2 enoyl Coenzyme A (CoA) hydratase alpha subunit Mitochondrial long chain L 3 hydroxyacyl Coenzyme A dehydrogenase alpha subunit Mitochondrial trifunctional enzyme alpha subunit Mitochondrial trifunctional protein alpha subunit MTPA Thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit TP ALPHA TP-alpha Trifunctional enzyme subunit alpha mitochondrial precursor |
Images
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Fig1:
Western blot analysis of HADH on different lysates with Rabbit anti-HADH antibody (R1411-4) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1411-4) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IF-Tissue Species: Human Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/100 |
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Fig3:
Application: IF-Tissue Species: Rat Site: kidney Sample: Paraffin-embedded section Antibody concentration: 1/100 |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HADH antibody (R1411-4) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1411-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HADH antibody (R1411-4) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1411-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-HADH antibody (R1411-4) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1411-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunocytochemistry analysis of HeLa cells labeling HADH with Rabbit anti-HADH antibody (R1411-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HADH antibody (R1411-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig8:
Immunocytochemistry analysis of HepG2 cells labeling HADH with Rabbit anti-HADH antibody (R1411-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HADH antibody (R1411-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig9:
Immunocytochemistry analysis of MCF7 cells labeling HADH with Rabbit anti-HADH antibody (R1411-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HADH antibody (R1411-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.