| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Staphylococcus aureus |
| Applications: | WB, ELISA, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 158 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within CRISPR-Cas9 aa 54-141. |
| Positive control: | CRISPR-Cas9 transfected 293 cells. |
| Recommended Dilutions:
WB ELISA IF-Cell |
1:2,000-1:5,000 1:10,000 1:200-1:500 |
| Uniprot #: | SwissProt: Q99ZW2 StreptococcusPyogenes |
| Alternative names: | Cas9 CRISPR-associated endonuclease Cas9/Csn1 CRISPR-Cas9/Csn1 csn1 SpyCas9 |
|
Fig1: Western blot analysis on CRISPR-Cas9 non transtected 293 cells (-) and transfected 293 cells (+). |
|
Fig2: Immunofluorescence analysis of CRISPR-Cas9 transfected 293 cells. The cell expressing CRISPR-Cas9 can be detected using rabbit anti- CRISPR-Cas9 (1/500) followed by FITC (1/1,000) goat anti-rabbit IgG. |