Lamin B1 Rabbit Polyclonal Antibody
cat.: R1508-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Lamin B1 aa 1-50 / 586. |
| Positive control: | Jurkat cell lysate, MCF-7 cell lysate, PC12 cell lysate, Wild-type Hela whole cell lysate. |
| Subcellular location: | Intermediate filament, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P20700 Human |
| Alternative names: | ADLD lamin B1 Lamin-B1 LMN LMN2 LMNB Lmnb1 LMNB1_HUMAN MGC111419 OTTHUMP00000159218 |
Images
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Fig1: Western blot analysis on different cell lysates using anti- Lamin B1 rabbit polyclonal antibodies. |
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Fig2:
All lanes: Western blot analysis of Lamin B1 with anti-Lamin B1 antibody (R1508-1) at 1/500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: Lamin B1 knockdown Hela whole cell lysate (10 µg). R1508-1 was shown to specifically react with Lamin B1 in wild-type Hela cells. Weakened band was observed when Lamin B1 knockdown sample was tested. Wild-type and Lamin B1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1508-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.