CCL3 / MIP-1 alpha Rabbit Polyclonal Antibody
cat.: R1510-10
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 10 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CCL3 aa 1-92.
Positive control: NK-92 cell lysate, THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P10147 Human
Alternative names: C C motif chemokine 3 CCL 3 CCL3 CCL3_HUMAN Chemokine (C C motif) ligand 3 Chemokine C C motif ligand 3 Chemokine ligand 3 G0/G1 switch regulatory protein 19 1 G0/G1 switch regulatory protein 19-1 G0S19 1 G0S19 1 protein Heparin binding chemotaxis protein L2G25B LD78 alpha LD78-alpha(4-69) LD78alpha Macrophage inflammatory protein 1 alpha Macrophage inflammatory protein 1-alpha macrophage inflammatory protein 1a MIP 1 alpha MIP 1A MIP-1-alpha MIP-1-alpha(4-69) MIP1 alpha MIP1A PAT 464.1 SCYA 3 SCYA3 SIS alpha SIS beta SIS-beta Small inducible cytokine A3 small inducible cytokine A3 (homologous to mouse Mip-1a) Small-inducible cytokine A3 Tonsillar lymphocyte LD78 alpha protein
Images
R1510-10_1.jpg Fig1: Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (R1510-10) at 1/1,000 dilution.

Lane 1: NK-92 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 10 kDa
Observed band size: 10 kDa

Exposure time: 1 minute; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.