PTPRF/LAR Rabbit Polyclonal Antibody
cat.: R1510-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 213 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein with Human PTPRF / LAR aa 134-650 / 1,907. |
| Positive control: | SiHa, human colon carcinoma tissue, human kidney tissue, A431. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:200-1:500 1:50-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P10586 Human |
| Alternative names: | FLJ43335 FLJ45062 FLJ45567 LAR LAR protein LARFN5C LARS LCA homolog Leukocyte antigen related (LAR) PTP receptor Leukocyte antigen related Leukocyte antigen related PTP receptor Leukocyte antigen related tyrosine phosphatase Leukocyte common antigen related Protein Tyrosine Phosphatase Receptor Type F Protein tyrosine phosphatase receptor type F polypeptide Ptprf PTPRF protein PTPRF_HUMAN Receptor linked protein tyrosine phosphatase LAR Receptor type tyrosine protein phosphatase F Receptor type tyrosine protein phosphatase F precursor Receptor-type tyrosine-protein phosphatase F |
Images
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Fig1: ICC staining of PTPRF/LAR in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1510-12, 1/500) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PTPRF/LAR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-12, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PTPRF/LAR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-12, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of PTPRF/LAR was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-12, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.