| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein with Human Rem2 aa 121-292 / 340. |
| Positive control: | A549, HepG2, rat brain tissue, rat kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:50-1:100 1:500 |
| Uniprot #: | SwissProt: Q8IYK8 Human |
| Alternative names: | cb111 chunp6884 etID309896.20 R2 reductase M2 polypeptide variant 1 reductase M2 polypeptide variant 2 reductase M2 polypeptide variant 3a reductase M2 polypeptide variant 3b reductase M2 polypeptide variant 3c reductase M2 polypeptide variant 3d Ribonucleoside-diphosphate reductase subunit M2 Ribonucleotide reductase M2 Ribonucleotide reductase M2 polypeptide Ribonucleotide reductase M2 subunit ribonucleotide reductase protein r2 class I Ribonucleotide reductase regulatory subunit M2 Ribonucleotide reductase small chain Ribonucleotide reductase small subunit Ribonucleotide reductase, R2 subunit RIR2_HUMAN RR2 RR2M RRM2 |
|
Fig1: ICC staining Rem2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Rem2 antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Rem2 antibody. Counter stained with hematoxylin. |
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Fig4: Flow cytometric analysis of HepG2 cells with Rem2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |