TMEM39a Rabbit Polyclonal Antibody
cat.: R1510-16
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human TMEM39a aa 475-488 / 488 Human. |
| Positive control: | Hela, PANC-1, human pancreas tissue, human colon tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:600 1:100 |
| Uniprot #: | SwissProt: Q9NV64 Human |
| Alternative names: | Hypothetical protein FLJ10902 Transmembrane protein 39A |
Images
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Fig1: Immunocytochemical staining of Hela cells using anti-TMEM39a rabbit polyclonal antibody. |
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Fig2: Immunocytochemical staining of PANC-1 cells using anti-TMEM39a rabbit polyclonal antibody. |
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Fig3: Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-TMEM39a rabbit polyclonal antibody. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TMEM39a antibody (R1510-16) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-16) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Hela cells with TMEM39a antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.