Myosin light chain kinase / MLCK Rabbit Polyclonal Antibody
cat.: R1510-18
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 211 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MLCK aa 36-85 / 1,914. |
| Positive control: | Hela, NIH/3T3, SHG-44, mouse smooth muscle tissue. |
| Subcellular location: | Cytoplasm, Cleavage furrow, Cell projection |
| Recommended Dilutions:
IF-Cell IF-Cell FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q15746 Human |
| Alternative names: | deglutamylated form DKFZp686I10125 EC 2.7.11.18 FLJ12216 Kinase related protein Kinase-related protein KRP MLCK MLCK1 MLCK108 MLCK210 MSTP083 MYLK MYLK_HUMAN MYLK1 Myosin light chain kinase Myosin light polypeptide kinase OTTHUMP00000180642 OTTHUMP00000180643 smMLCK smooth muscle Smooth muscle myosin light chain kinase Telokin |
Images
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Fig1: Immunocytochemical staining of Hela cells using anti-MLCK rabbit polyclonal antibody. |
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Fig2: Immunocytochemical staining of NIH/3T3 cells using anti-MLCK rabbit polyclonal antibody. |
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Fig3: Immunocytochemical staining of SHG-44 cells using anti-MLCK rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MLCK rabbit polyclonal antibody. |
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Fig5: Flow cytometric analysis of N2A cells with MLCK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.