Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse |
Applications: | IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human MLCK aa 36-85 / 1,914. |
Positive control: | Hela, NIH/3T3, SHG-44, mouse smooth muscle tissue. |
Subcellular location: | Cytoplasm, Cleavage furrow, Cell projection |
Recommended Dilutions:
IF-Cell IF-Cell FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: Q15746 Human |
Alternative names: | deglutamylated form DKFZp686I10125 EC 2.7.11.18 FLJ12216 Kinase related protein Kinase-related protein KRP MLCK MLCK1 MLCK108 MLCK210 MSTP083 MYLK MYLK_HUMAN MYLK1 Myosin light chain kinase Myosin light polypeptide kinase OTTHUMP00000180642 OTTHUMP00000180643 smMLCK smooth muscle Smooth muscle myosin light chain kinase Telokin |
Fig1: Immunocytochemical staining of Hela cells using anti-MLCK rabbit polyclonal antibody. | |
Fig2: Immunocytochemical staining of NIH/3T3 cells using anti-MLCK rabbit polyclonal antibody. | |
Fig3: Immunocytochemical staining of SHG-44 cells using anti-MLCK rabbit polyclonal antibody. | |
Fig4: Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MLCK rabbit polyclonal antibody. |
Fig5: Flow cytometric analysis of N2A cells with MLCK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |