MLCK Rabbit Polyclonal Antibody
cat.: R1510-18
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Isotype: IgG
Immunogen: Synthetic peptide within Human MLCK aa 36-85 / 1,914.
Positive control: Hela, NIH/3T3, SHG-44, mouse smooth muscle tissue.
Subcellular location: Cytoplasm, Cleavage furrow, Cell projection
Recommended Dilutions:
  IF-Cell
  IF-Cell
  FC

1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q15746 Human
Alternative names: deglutamylated form DKFZp686I10125 EC 2.7.11.18 FLJ12216 Kinase related protein Kinase-related protein KRP MLCK MLCK1 MLCK108 MLCK210 MSTP083 MYLK MYLK_HUMAN MYLK1 Myosin light chain kinase Myosin light polypeptide kinase OTTHUMP00000180642 OTTHUMP00000180643 smMLCK smooth muscle Smooth muscle myosin light chain kinase Telokin
Images
R1510-18_1.jpg Fig1: Immunocytochemical staining of Hela cells using anti-MLCK rabbit polyclonal antibody.
R1510-18_2.jpg Fig2: Immunocytochemical staining of NIH/3T3 cells using anti-MLCK rabbit polyclonal antibody.
R1510-18_3.jpg Fig3: Immunocytochemical staining of SHG-44 cells using anti-MLCK rabbit polyclonal antibody.
R1510-18_4.jpg Fig4: Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MLCK rabbit polyclonal antibody.
R1510-18_5.jpg Fig5: Flow cytometric analysis of N2A cells with MLCK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.