Caspase-1 Rabbit Polyclonal Antibody
cat.: R1510-23
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Caspase-1 aa 162-205. |
| Positive control: | Jurkat, human lung tissue, human liver tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P29466 Human |
| Alternative names: | Caspase1 CASP-1 CASP1 CASP1_HUMAN Caspase1 Caspase 1 Caspase-1 subunit p10 ICE IL-1 beta-converting enzyme IL-1BC IL1 beta converting enzyme IL1B convertase Interleukin 1 beta convertase Interleukin 1B converting enzyme Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme p45 |
Images
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Fig1:
Immunocytochemistry analysis of Jurkat cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (R1510-23) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (R1510-23) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- Caspase-1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Caspase-1 antibody. Counter stained with hematoxylin. |
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Fig4: Flow cytometric analysis of Jurkat cells with Caspase-1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.