Smad3 Rabbit Polyclonal Antibody
cat.: R1510-24
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human SMAD3. |
| Positive control: | Hela, HCT116, HepG2, human colon cancer tissue, human lung carcinoma tissue, human breast carcinoma tissue, human liver tissue, human stomach carcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus, endoplasmic reticulum |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:50-1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: P84022 Human |
| Alternative names: | DKFZP586N0721 DKFZp686J10186 hMAD 3 hMAD-3 hSMAD3 HSPC193 HST17436 JV15 2 JV15-2 JV152 LDS1C LDS3 MAD (mothers against decapentaplegic Drosophila) homolog 3 MAD homolog 3 Mad homolog JV15 2 Mad protein homolog MAD, mothers against decapentaplegic homolog 3 Mad3 MADH 3 MADH3 MGC60396 Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3 SMA and MAD related protein 3 SMAD 3 SMAD SMAD family member 3 SMAD, mothers against DPP homolog 3 Smad3 SMAD3_HUMAN |
Images
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Fig1: Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody. . |
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Fig2: Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody. . |
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Fig3: Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody. . |
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Fig4: Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. . |
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Fig5: Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-SMAD3 rabbit polyclonal antibody. . |
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Fig6: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-SMAD3 rabbit polyclonal antibody. . |
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Fig7: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. . |
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Fig8: Immunohistochemical analysis of paraffin- embedded human stomach carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. . |
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Fig9: Flow cytometric analysis of Hela cells with SMAD3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.