Smad3 Rabbit Polyclonal Antibody
cat.: R1510-24
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human SMAD3.
Positive control: Hela, HCT116, HepG2, human colon cancer tissue, human lung carcinoma tissue, human breast carcinoma tissue, human liver tissue, human stomach carcinoma tissue.
Subcellular location: Cytoplasm, Nucleus, endoplasmic reticulum
Recommended Dilutions:
  IF-Cell
  IHC-P
  FC
  WB

1:50-1:200
1:50-1:200
1:50-1:100
1:500-1:1,000
Uniprot #: SwissProt: P84022 Human
Alternative names: DKFZP586N0721 DKFZp686J10186 hMAD 3 hMAD-3 hSMAD3 HSPC193 HST17436 JV15 2 JV15-2 JV152 LDS1C LDS3 MAD (mothers against decapentaplegic Drosophila) homolog 3 MAD homolog 3 Mad homolog JV15 2 Mad protein homolog MAD, mothers against decapentaplegic homolog 3 Mad3 MADH 3 MADH3 MGC60396 Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3 SMA and MAD related protein 3 SMAD 3 SMAD SMAD family member 3 SMAD, mothers against DPP homolog 3 Smad3 SMAD3_HUMAN
Images
R1510-24_1.jpg Fig1: Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody. .
R1510-24_2.jpg Fig2: Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody. .
R1510-24_3.jpg Fig3: Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody. .
R1510-24_4.jpg Fig4: Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .
R1510-24_5.jpg Fig5: Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-SMAD3 rabbit polyclonal antibody. .
R1510-24_6.jpg Fig6: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-SMAD3 rabbit polyclonal antibody. .
R1510-24_7.jpg Fig7: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .
R1510-24_8.jpg Fig8: Immunohistochemical analysis of paraffin- embedded human stomach carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .
R1510-24_9.jpg Fig9: Flow cytometric analysis of Hela cells with SMAD3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.