nNOS Rabbit Polyclonal Antibody
cat.: R1510-28
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC, IHC-Fr |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 161 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human nNOS. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, mouse colon tissue, rat colon tissue, SHG-44, SK-BR-3, HepG2, N2A. |
| Subcellular location: | Cell membrane, Cell projection |
| Recommended Dilutions:
WB IF-Cell IHC-P FC IHC-Fr |
1:1,000 1:50-1:200 1:10,000-1:50,000 1:50-1:100 1:5,000 |
| Uniprot #: | SwissProt: P29475 Human | Q9Z0J4 Mouse | P29476 Rat |
| Alternative names: | 2310005C01Rik BNOS Constitutive NOS EC 1.14.13.39 IHPS 1 IHPS1 N-NOS NC-NOS neuronal Nitric Oxide Synthase Neuronal NOS Nitric oxide synthase , neuronal, included Nitric oxide synthase 1 (neuronal) Nitric oxide synthase 1 Nitric oxide synthase, brain Nitric oxide synthase, penile neuronal, included NNOS NO NOS 1 NOS NOS type I NOS-I NOS1 NOS1_HUMAN Peptidyl-cysteine S-nitrosylase NOS1 |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse colon tissue with Rabbit anti-nNOS antibody (R1510-28) at 1/5,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (R1510-28, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Western blot analysis of nNOS on different lysates with Rabbit anti-nNOS antibody (R1510-28) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 161 kDa Observed band size: 161 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-nNOS antibody (R1510-28) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-28) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-nNOS antibody (R1510-28) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-28) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Western blot analysis on SHG-44(1) and mouse brain(2) lysates using anti-nNOS rabbit polyclonal antibody. |
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Fig6: Immunocytochemical staining of SK-BR-3 cells using anti-nNOS rabbit polyclonal antibody. |
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Fig7: Immunocytochemical staining of HepG2 cells using anti-nNOS rabbit polyclonal antibody. |
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Fig8: Flow cytometric analysis of N2A cells with nNOS antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.