nNOS Rabbit Polyclonal Antibody
cat.: R1510-28
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 160kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human nNOS. |
| Positive control: | SHG-44, SK-BR-3, HepG2, mouse lung tissue, human lung tissue, mouse testis tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue. |
| Subcellular location: | Cell membrane, Cell projection |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P29475 Human | Q9Z0J4 Mouse | P29476 Rat |
| Alternative names: | 2310005C01Rik BNOS Constitutive NOS EC 1.14.13.39 IHPS 1 IHPS1 N-NOS NC-NOS neuronal Nitric Oxide Synthase Neuronal NOS Nitric oxide synthase , neuronal, included Nitric oxide synthase 1 (neuronal) Nitric oxide synthase 1 Nitric oxide synthase, brain Nitric oxide synthase, penile neuronal, included NNOS NO NOS 1 NOS NOS type I NOS-I NOS1 NOS1_HUMAN Peptidyl-cysteine S-nitrosylase NOS1 |
Images
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Fig1: Western blot analysis on SHG-44(1) and mouse brain(2) lysates using anti-nNOS rabbit polyclonal antibody. |
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Fig2: Immunocytochemical staining of SK-BR-3 cells using anti-nNOS rabbit polyclonal antibody. |
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Fig3: Immunocytochemical staining of HepG2 cells using anti-nNOS rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin- embedded human lung tissue using anti-nNOS rabbit polyclonal antibody. |
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Fig5: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-nNOS rabbit polyclonal antibody. |
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Fig6: Immunohistochemical analysis of paraffin- embedded mouse lung tissue using anti-nNOS rabbit polyclonal antibody. |
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Fig7: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-nNOS rabbit polyclonal antibody. |
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Fig8: Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-nNOS rabbit polyclonal antibody. |
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Fig9: Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-nNOS rabbit polyclonal antibody. |
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Fig10: Flow cytometric analysis of N2A cells with nNOS antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.