Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 85kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within N-terminal human Hsp90 alpha. |
Positive control: | Hela, PC-12, NIH/3T3, HepG2, C2C12, mouse kidney tissue, human kidney tissue, mouse testis tissue. |
Subcellular location: | Nucleus, Cytoplasm, Melanosome, Cell membrane, Mitochondrion. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P07900 Human | P07901 Mouse | P82995 Rat |
Alternative names: | EL52 epididymis luminal secretory protein 52 Heat shock 86 kDa heat shock 90kD protein 1, alpha Heat shock 90kD protein 1, alpha like 4 heat shock 90kD protein, alpha-like 4 Heat shock 90kDa protein 1 alpha Heat shock protein 90kDa alpha (cytosolic) class A member 1 Heat shock protein HSP 90-alpha HS90A_HUMAN HSP 86 HSP86 Hsp89 HSP89A Hsp90 HSP90A HSP90AA1 HSP90ALPHA HSP90N HSPC1 HSPCA HSPCAL1 HSPCAL4 HSPN LAP 2 LAP2 lipopolysaccharide-associated protein 2 LPS-associated protein 2 Renal carcinoma antigen NY-REN-38 |
Fig1:
Western blot analysis on different cell lysates using anti-HSP90 alpha rabbit polyclonal antibody. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: PC-12 Lane 4: NIH/3T3 |
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Fig2: Immunocytochemical staining of HepG2 cells using anti-HSP90 alpha rabbit polyclonal antibody. | |
Fig3: Immunocytochemical staining of C2C12 cells using anti-HSP90 alpha rabbit polyclonal antibody. | |
Fig4: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-HSP90 alpha rabbit polyclonal antibody. |
Fig5: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-HSP90 alpha rabbit polyclonal antibody. | |
Fig6: Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-HSP90 alpha rabbit polyclonal antibody. | |
Fig7: Flow cytometric analysis of Hela cells with HSP90 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |