Prohibitin(Mitochondrial Marker) Rabbit Polyclonal Antibody
cat.: R1510-30
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 30kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Prohibitin. |
| Positive control: | Hela, HepG2, C2C12, F9, PC12, Jurkat, human kidney tissue, mouse liver tissue, human liver carcinoma tissue, mouse kidney tissue, mouse heart tissue. |
| Subcellular location: | Mitochondrion inner membrane, Nucleus, Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P35232 Human | P67778 Mouse | P67779 Rat |
| Alternative names: | Epididymis luminal protein 215 Epididymis secretory sperm binding protein Li 54e HEL 215 HEL S 54e PHB PHB_HUMAN PHB1 Prohibitin |
Images
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Fig1:
Western blot analysis on different cell lysates using anti-Prohibitin rabbit polyclonal antibody. Positive control: Lane 1: F9 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: PC-12 |
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Fig2: Immunocytochemical staining of Hela cells using anti-Prohibitin rabbit polyclonal antibody. |
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Fig3: Immunocytochemical staining of HepG2 cells using anti-Prohibitin rabbit polyclonal antibody. |
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Fig4: Immunocytochemical staining of C2C12 cells using anti-Prohibitin rabbit polyclonal antibody. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Prohibitin(Mitochondrial Marker) antibody (R1510-30) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-30) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Prohibitin rabbit polyclonal antibody. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Prohibitin(Mitochondrial Marker) antibody (R1510-30) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-30) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8: Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-Prohibitin rabbit polyclonal antibody. |
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Fig9: Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-Prohibitin rabbit polyclonal antibody. |
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Fig10:
Flow cytometric analysis of HepG2 cells labeling Prohibitin(Mitochondrial Marker). Cells were fixed and permeabilized. Then stained with the primary antibody (R1510-30, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.