PP2A(alpha+beta) Rabbit Polyclonal Antibody
cat.: R1510-31
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to of Human PP2A(alpha+beta) aa 260-309 / 309. |
| Positive control: | Hela, A431, HepG2, PC12, human breast carcinoma tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Chromosome |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P67775 Human | P63330 Mouse | P63331 Rat |
| Alternative names: | PP2A A PP2A alpha PP2A B PP2A beta PP2A-alpha PP2AA_HUMAN PP2Ac PP2CB PPP2CA PPP2CB Protein phosphatase 2 catalytic subunit alpha isoform Protein phosphatase 2 catalytic subunit beta isoform Replication protein C RP C RP-C Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform Serine/threonine protein phosphatase 2A catalytic subunit beta isoform Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
Images
|
Fig1:
Western blot analysis on different cell lysates using anti-PP2A(alpha+beta) rabbit polyclonal antibody. Positive control: Lane 1: A431 Lane 2: HepG2 Lane 3: F9 Lane 4: PC-12 |
|
Fig2: Immunocytochemical staining of Hela cells using anti-PP2A(alpha+beta) rabbit polyclonal antibody. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PP2A(alpha+beta) antibody (R1510-31) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-31) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.