MSH2 Rabbit Polyclonal Antibody
cat.: R1510-32
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MSH2 aa 21-70 / 934. |
| Positive control: | Hela. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
IF-Cell FC |
1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P43246 Human | P43247 Mouse | P54275 Rat |
| Alternative names: | BAT26 COCA 1 COCA1 DNA mismatch repair protein Msh2 FCC 1 FCC1 hMSH2 HNPCC 1 HNPCC HNPCC1 LCFS2 MSH 2 Msh2 MSH2_HUMAN MutS homolog 2 MutS homolog 2 colon cancer nonpolyposis type 1 MutS protein homolog 2 |
Images
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Fig1: ICC staining of MSH2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1510-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: Flow cytometric analysis of MSH2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-32, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.