Anti-MSH2 antibody
cat.: R1510-32
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 105 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human MSH2.
Positive control: Hela.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  ICC
  FC

1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P43246 Human
Alternative names: BAT26 COCA 1 COCA1 DNA mismatch repair protein Msh2 FCC 1 FCC1 hMSH2 HNPCC 1 HNPCC HNPCC1 LCFS2 MSH 2 Msh2 MSH2_HUMAN MutS homolog 2 MutS homolog 2 colon cancer nonpolyposis type 1 MutS protein homolog 2
Images
R1510-32_1.jpg Fig1: ICC staining of MSH2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1510-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
R1510-32_2.jpg Fig2: Flow cytometric analysis of MSH2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-32, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.